Occurrence - Code 21270

Occurrence Details
Date 20/07/2012 -- 28/02/2015
Method Transect sampling
Description Samples of infected plants showing symptoms of leaf / folair / sheath blight were collected from six to eight disease foci per row (~10 m between each focus) in a total of five rows per field with the goal of obtaining approximately 30 - 40 isolates per field population, keeping a single isolate per focus. Isolation and preservation of the fungal isolates were performed as previously described. Briefly, isolations were made by placing fragments of infected leaves into Petri dishes containing modified Ko & Hora selective medium and incubating at 25°C in the dark. Pure cultures were established by transferring hyphal fragments to potato dextrose agar medium containing 50 μg/mL chloramphenicol and streptomycin. Sclerotia from five-day-old cultures were transferred to 1.8-mL cryotubes containing anhydrous silica gel for long-term storage at -20°C.
Additional observations
Occurrence author
Name Paulo Ceresini
Address _
Project A origem de populações emergentes do patógeno da queima da folha da Brachiaria spp. (Rhizoctonia solani AG-1) na Amazônia e seu potencial de adaptação a outros agroecossistemas brasileiros
Website _
Reference A etiologia complexa de doenças causadas por fungos do gênero Rhizoctonia em Brachiaria e em culturas simpátricas de arroz, feijão-caupi ou soja na Amazônia, nos Cerrados Brasileiros e no Vale do Paraíba
Date & user March 2, 2015 by user (pauloceresini)
Municipality Mucajaí, RR
Location Agricultural area with the forage crop Brachiaria brizantha cv. Piatã
Environment Terrestrial
GPS Precision 7.0
Occurrence Precision Occurrence Place
Occurrence Extension 1000 m
Ecosystems Agroecossistema
Habitats Cultura anual
Microhabitats Folha
Taxonomic Information
Content 13 specimens
Taxonomic Groups None

Species List